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easysep mouse hsc isolation kit  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc easysep mouse hsc isolation kit
    Easysep Mouse Hsc Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/easysep mouse hsc isolation kit/product/STEMCELL Technologies Inc
    Average 90 stars, based on 1 article reviews
    easysep mouse hsc isolation kit - by Bioz Stars, 2026-04
    90/100 stars

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    easysep mouse hsc isolation kit  (STEMCELL Technologies Inc)
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    STEMCELL Technologies Inc easysep mouse hsc isolation kit
    Easysep Mouse Hsc Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse hsc isolation kit  (STEMCELL Technologies Inc)
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    A–C) Bone marrow was isolated from donor Rag1−/− mice <t>and</t> <t>HSCs</t> were purified by negative selection of lineage-positive cells. Purified HSCs were left untransduced or transduced 1–3 times with DO11 or GUCY2C TCRs. A) <t>HSC</t> expansion ex vivo. B) Transduced HSCs remained lineage negative (mature lineage markers B220, Ter119, CD4, and Gr-1 shown here) following retroviral transduction and expansion, comparable to HSCs stained directly ex vivo. C) HSC transduction efficiency following 1, 2, or 3 applications of TCR retrovirus (1x, 2x or 3x). Values indicate percentage of GFP+ cells. Data are representative of 3 experiments.
    Mouse Hsc Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    mouse hsc isolation kit - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    mouse hsc isolation kit  (Becton Dickinson)
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    Becton Dickinson mouse hsc isolation kit
    A–C) Bone marrow was isolated from donor Rag1−/− mice <t>and</t> <t>HSCs</t> were purified by negative selection of lineage-positive cells. Purified HSCs were left untransduced or transduced 1–3 times with DO11 or GUCY2C TCRs. A) <t>HSC</t> expansion ex vivo. B) Transduced HSCs remained lineage negative (mature lineage markers B220, Ter119, CD4, and Gr-1 shown here) following retroviral transduction and expansion, comparable to HSCs stained directly ex vivo. C) HSC transduction efficiency following 1, 2, or 3 applications of TCR retrovirus (1x, 2x or 3x). Values indicate percentage of GFP+ cells. Data are representative of 3 experiments.
    Mouse Hsc Isolation Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A–C) Bone marrow was isolated from donor Rag1−/− mice and HSCs were purified by negative selection of lineage-positive cells. Purified HSCs were left untransduced or transduced 1–3 times with DO11 or GUCY2C TCRs. A) HSC expansion ex vivo. B) Transduced HSCs remained lineage negative (mature lineage markers B220, Ter119, CD4, and Gr-1 shown here) following retroviral transduction and expansion, comparable to HSCs stained directly ex vivo. C) HSC transduction efficiency following 1, 2, or 3 applications of TCR retrovirus (1x, 2x or 3x). Values indicate percentage of GFP+ cells. Data are representative of 3 experiments.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: TCR Retrogenic Mice as a Model to Map Self-Tolerance Mechanisms to the Cancer Mucosa Antigen Guanylyl Cyclase C (GUCY2C)

    doi: 10.4049/jimmunol.1801206

    Figure Lengend Snippet: A–C) Bone marrow was isolated from donor Rag1−/− mice and HSCs were purified by negative selection of lineage-positive cells. Purified HSCs were left untransduced or transduced 1–3 times with DO11 or GUCY2C TCRs. A) HSC expansion ex vivo. B) Transduced HSCs remained lineage negative (mature lineage markers B220, Ter119, CD4, and Gr-1 shown here) following retroviral transduction and expansion, comparable to HSCs stained directly ex vivo. C) HSC transduction efficiency following 1, 2, or 3 applications of TCR retrovirus (1x, 2x or 3x). Values indicate percentage of GFP+ cells. Data are representative of 3 experiments.

    Article Snippet: Briefly, Rag1 − /− mice were injected i.p. with 150 mg/kg of 5-fluorouracil (Millipore Sigma); bone marrow was harvested 72 h later and murine HSCs were purified using mouse HSC isolation kit and EasySep magnet (STEMCELL, Vancouver, BC, Canada).

    Techniques: Isolation, Purification, Selection, Ex Vivo, Transduction, Staining